Course prerequisites:
Biology, Health Science, and Molecular Biology majors must have taken BIOL 108/109. Other majors must have taken BIOL 108/109 or have consent of the instructor.

Other Information:
If you have a documented learning, psychological, or physical disability, you may be eligible for reasonable academic accommodations or services. To request accommodations or services, contact the Academic Resource Center in Kindlon Hall, Room 249. All students are expected to fulfill essential course requirements. The University will not waive any essential skill or requirement of a course or degree program.

Attendance Policy:
Attendance at lectures is expected; attendance at all exams is required. Attendance will be taken, and it is in the student's best interest to attend class. Each student will be allowed 6 absences from class. Excessive absences will result in the reduction of that student's final, cumulative grade by one letter grade. Students having difficulty meeting the attendance requirement should speak to Dr. Tischler as soon as the problem arises. Absence at exams will result in a grade of zero for that exam unless arrangements are made with the instructor at least one day prior to the day of the exam.

Course Drop Date:
The last day to drop a course at Benedictine U. is Nov. 2.

Academic Honesty Policy:
All students are required to read and abide by the Honesty Statement in the Student Handbook. Any instance of an activity that constitutes cheating as outlined in the statement of Academic Policy in the Student Handbook will result in a grade of F for this course. Plagiarism (using someone's ideas, not just exact words, without crediting that person) is considered cheating. Any instance of cheating will be reported to the Dean of Students, the student's advisor, and the Health Sciences Recommendation Committee.

Lab Period	Exercise Description	Exercise No.
8/30	Check in and introduction Use of the microscope Simple staining Broth culture Streak plate#	pg. x 1 12 7 9
9/6	Broth culture (cont) Streak plate (cont) Microbial flora of the oral cavity Gram stain# Demonstration of phase contrast microscopy Pipetting and dilution of dye	7 9 3 13 4 16
9/13	Gram stain (cont) Applied microbiology of foods beer, pickles enrichment of Lactobacillus from yogurt Turbidometric estimation of bacterial growth*	13 handout 60 46 19
9/20	Applied microbiology (cont) Gram stain of pickle cultures Gram stain of yogurt enrichments, isolation Bacterial growth (cont) Degradation of carbohydrates Degradation of proteins and amino acids Urea hydrolysis bring in soil sample for next class period	handout 46 19 35 36 37
9/27	No class -- study for your other classes
10/4	Confirmation of yogurt isolate Degradation of carbohydrates (cont) Degradation of proteins and amino acids (cont) Urea hydrolysis (cont) Respiratory enzymes Endospore stain Acid-fast stain Soil enrichments Bacillus isolation* Pseudomonad isolation* Isolation of an anaerobe* Isolation of an antibiotic producer*	46 35 36 37 38 14 handout 43 44 handout 33
10/11	Lab quiz Soil enrichments (cont) Bacillus isolation* Pseudomonad isolation* Isolation of an anaerobe* Isolation of an antibiotic producer* Effect of temperature on growth bring in disinfectant for next class	43 44 handout 33 20
10/18	Soil enrichments (cont) Effect of temperature on growth (cont) Antiseptic and disinfectant action Isolation of Staphylococcus Isolation of Streptococcus Kirby-Bauer technique for antibiotic action	20 30 45 70 71
10/25	Soil enrichments (cont) Antiseptic and disinfectant action (cont) Staphylococcus isolation (cont) Streptococcus isolation (cont) Kirby-Bauer (cont) Microbial Analysis of food*	30 45 70 71 handout
11/1	Soil enrichments (cont) Antibiotic action (cont) Staphylococcus isolation (cont) Streptococcus isolation (cont) Microbial analysis of food (cont) Unknowns*	71 45 70 handout
11/8	Unknowns
11/15	Unknowns
11/22	No class -- Thanksgiving Day Eat turkey and watch parades and football games
11/29	Unknowns	handout
12/6	Final lab quiz Applied Microbiology finish up unknowns Check-out

#lab skill; *lab write-up

Examinations:
All exams will be a combination of objective questions (multiple choice, fill in the blank, labeling diagrams, matching, etc.), short answer, and essay questions. Exams will be primarily over lecture material; however students are responsible for material in the textbook if the topic was covered in lecture. Since the lectures will correspond largely to material in the book, it is in the best interest of the student to read the text.

Final Exam:
The final exam will be comprehensive and will cover both lecture and laboratory material.

Laboratory Pop Quizzes:
Pop quizzes may cover both

1. the readings from the lab manual due that week, and
   
2. the readings or techniques or procedures covered since the last pop quiz or lab quiz.
   

Be assured that there will be pop quizzes if it appears that students are not coming to lab prepared.

Practical Skills:
There will be no formal "lab practical" exams in this class. However, students are expected to master certain basic microbiology laboratory skills. Each student will have to demonstrate to the instructor the mastery of those skills before the end of the semester.

Basis of Grading:

Assignment	Date	Points
Exam #1	9/24	100
Exam #2	10/19	100
Exam #3	11/16	100
Final Exam	Friday, 12/14; 10:15 am	200
Lab Quiz #1	10/11	30
Final Lab Quiz	12/6	30
Lab assignments, pop quizzes	.	30
Practical lab skills	.	15
Unknowns	.	25
lab attendance, courtesy, and cooperation	.	20
Total	.	650

Grades will be assigned on the following basis:

Dr. Tischler's Home Page

This page was last updated on August 24, 2001 by M. Tischler.