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BenU Faculty since 2007
Ph.D. - Physiology, University of Illinois at Chicago, IL (1999)
M.Phil - Crystallography and Biophysics, University of Madras, India (1991)
M.S. - Biophysics, University of Madras, India (1990)
B.S. - Physics, University of Madras, India (1988)

Courses Taught
Undergraduate Level
BIOL 258 – Human Physiology
BIOL 259 – Human Physiology Lab
BIOL 391 – Pathophysiology: Special topics
BIOL 340 – Cell Biology
BIOL 150 – Biology of Women

Graduate Level
EXPH 591/HLSC 392 – Exercise Biochemistry
EXPH 560/NUTR 560/HLSC 360 – Advanced Cardiovascular and Respiratory Physiology 

Awards and Recognitions:
Dean's Award for Teaching Excellence by an Adjunct Faculty 2010

Research Area
My research interests are to elucidate, at a molecular level, the processes that modulate ion transport across epithelial cells. Epithelia, such as those lining the gastrointestinal tract, are multi-faceted and complex. For example, salt and water transport along the length of the intestine needs to be tightly regulated for normal function, and aberrations can result in some of the most devastating forms of diarrhea in a variety of species.  Regulation of ion and water transport is also important in the mammary epithelia and aid with milk secretion. Therefore, studying the cellular and molecular regulation of ion transport by hormones, chemicals and drugs is important in defining fundamental physiological processes of fluid absorption and secretion, which can pertain to epithelial cells from mammalian mammary to the colonic epithelial cells. Model systems such as the human colon carcinoma cell-line (T84 cells) and mouse mammary epithelial cells (HC11) are used in these studies.

Current Research Projects

  • Does bile acid modulate tight junction to increase paracellular permeability? Cells will be grown on inserts to form a monolayer, challenged with various doses of bile acid. Transepithelial resistance will be measured using anelectrical resistance system and paracellular permeability measured via mucosal to basolateral flux of 10-kDa fluorescence tagged dextran.
  • Is the drop in resistance due to cytotoxic effects of bile acids? This will be assessed by measuring lactate dehydrogenase activity in the supernatant of cells treated with various doses of bile acids. Cytotoxicity is indicated if the LDH activity is increases in the solution. This is measured using a colorimetric assay.

Books/Chapters Published

  1. Integrated Systems Physiology: Intestinal Absorption and Secretion by Rao, MC,  Sarathy J, and Ao M, Publisher: Morgan and Claypool Life sciences, NJ. e-Book.
  2. Venkatasubramanian J, Rao MC, and Sellin JH. Intestinal electrolyte absorption and secretion in Sleisenger and Fordtran's Gastrointestinal and Liver Disease: Pathophysiology, Diagnosis, Management, 9th edition, Chapter 99, (2010).

Papers Published (Graduate and undergraduate students and Research Specialists mentored are italicized)

  1. Anantamongol U, Ao M, Sarathy J (nee Venkatasubrmanian), Devi S, Krishnamra N, and Rao MC. Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia. Journal of Signal Transduction, Volume 2012 (2012), Article ID 192142, 15 pages. doi: 10.1155/2012/192142.
  2. Ao M, Venkatasubramanian J, Ganesan Syed, Benya RV and Rao MC. Lubiprostone Activates Cl Secretion by Increasing CFTR in Apical Membrane in the Human Colon Carcinoma Cell Line, T84, Digestive Diseases in Science, 2011, 56(2):339-51. 
  3. Liu H, Singla A, Ao M, Gill RK, Venkatasubramanian J, Rao MC, Alrefai WA, Dudeja PK. Calcitonin Receptor-Mediated CFTR Activation in Human Intestinal Epithelial Cells J Cell Mol Med.; 2011; 10.1111/j.1582-4934.
  4. Venkatasubramanian, J., Ao, M., and Rao M.C. Ion transport in the small intestine – Current Opinions     Gastroenterology, 2010 Mar;26(2):123-8.
  5. Maria A Carlos, Chimnoya Nwagwu, Mei Ao, J Venkatasubramanian, Roli Prasad, Shamim Khan Chowdhury, Dharmapuri Vidyasagar, Mrinalini C Rao, Epidermal Growth Factor (EGF) stimulates chloride transport in primary cultures of weanling and adult rabbit colonocytes, Journal of Pediatric Gastroenterology and Nutrition, 44: 300-311, 2007.
  6. Prasad, R., Venkatasubramanian, J., Amde, M., and Rao, M.C. Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon. Digestive Disease Sciences 49:1318-1326, 2004.
  7. Venkatasubramanian, J., Nataraja, S. G., Carlos, M., Skaluba, S., Rasenick, M.M.R. and Rao, M. C. Differences in calcium signaling underlie age-specific effects of secretagogues on colonic chloride transport, 2001, American Journal Physiology: Cell Physiology, 280, 646-658. (Among Top 50 most read paper in month of Oct 2008)
  8. Venkatasubramanian, J., Sahi, J., and Rao, M.C.  Ion transport during growth and differentiaton, 2000, Annals of New York Academy of Sciences, 915, 357-372.
  9. G. N. Desai, J. Sahi, P. M. Reddy, J. Venkatasubramanian, D. Vidyasagar, M. C. Rao., Chloride transport in primary cultures of rabbit colonocytes at different stages of development, 1996,  Gastroenterology; 111, 1541-1550.

Peer-reviewed Abstracts

  1. *Ao M, Sarathy J (nee Venkatasubramanian), Domingue J, Mathew J, Alrefai WA, Rao MC, ^Contributed equally, Chenodeoxycholic Acid (CDCA) Stimulates  Cl- Secretion Across the Apical Membrane of Human Colonic Epithelial Cells, T84, via  cAMP Signaling and Increases in the Phosphorylation of CFTR., May 2012, Poster Presentation in Digestive Diseases Week, San Diego 
  2. *J Sarathy (nee Venkatasubramanian), M Ao, J Syed, W. A Alrefai, MC Rao, Bile acid-induced Cl secretion involves cytoskeleton and activation of CFTR in colonic epithelial cells, T84., May 2011, Poster Presentation in Digestive Diseases Week, Chicago.

Jayashree Sarathy, Ph.D.

Birck 339 | 630-829-6580


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